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Cell Biology

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From Harvesting to Cell Manipulation

Cell Biology is a branch of biology that studies cells - the organelles they contain, their function, their physiological properties, their life cycle, the interactions with their environment etc. Cell Biology basic research can be divided into several subfields e.g. the study of cell metabolism, the study of cell genetics and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell communication and signaling. Research in cell biology overlaps to a great extent with other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology.

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Who isn’t aware that acetone, ethanol, and other volatile liquids start to drip out of the pipette tip directly after aspiration? Probably, every one of us has experienced this. Are you wondering if this issue can be solved with pipetting “hacks” like “working as fast as possible” while “placing the tubes very close to each other to avoid chemical loss and spillage”? The answer is: probably not! Even when pipetting volatile liquids faster, you’re still likely to lose accuracy and precision. Yet all is not lost – by just adding some small changes in your pipetting techniques, and the right choice of pipette type can help you overcome these daily challenges!
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In this article, we look at the question whether or not water-jacketed incubators can provide a higher relative humidity and the rationale behind it.

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Starting an experiment means asking many questions. Which material is needed? Which samples are used? Which conditions are necessary, e.g., growth? How long is the whole application? Do I have to check on the experiment at weekends, or at night? One question is often forgotten, but is of no less importance. Which liquids are used during the application and how are they pipetted?
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La semina cellulare è solitamente il primo passo del protocollo e una procedura standard negli esperimenti su cellule. Un protocollo di semina cellulare corretto e standardizzato è un fattore critico per ottenere risultati sperimentali riproducibili. La sfida principale in questa fase è quella di ottenere e mantenere numeri comparabili di cellule in tutti gli esperimenti ripetuti.
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Contamination is one of the worst fears for scientists working in a cell culture laboratory. Biological contamination in cell culture is nearly always associated with experimental repeats, project delays, and sometimes even with lab closures or the loss of irreplaceable cells – wasting valuable time and precious resources.
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Cell thawing is an essential and sometimes undervalued element in all areas of cell-based research and production. A standardized cryopreservation process – including a standardized cell thawing protocol – helps to use cell stocks optimally for reproducible, reliable results.
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It cannot be ignored: The workload in labs is constantly growing. 25 years ago, working with 96-well microplates may have been progressive but nowadays an increasing number of labs are changing to 384-well plate format. Super, now you have four 96-well plates on a single plate – just with wells in miniature format. But what is the advantage? When comparing 384-well plate vs 96-well plate formats, the differences in efficiency and costs become clear. Let´s take a closer look at the advantages of 384-well plates.
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In vitro, stem cells are traditionally grown on a 2D surface such as a T-flask or a cell culture plate. However, as this approach offers limited options for scaling up, when it is important to be able to grow larger numbers of cells, bioreactors are often preferable.
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The absorbance measurement of a nucleic acid solution at only four wavelengths is sufficient to determine its concentration and to obtain an insight into its purity.
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Have you ever wondered how mycoplasma get into your cell culture? Learn more about potential sources and helpful ressources in this article.
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